4/5/2023 0 Comments Chimera software![]() ![]() PCR techniques, although commonly used and arguably the most powerful and accurate means of characterizing bacterial diversity also generate chimeric sequences. Net GUI-based application and define the stringency to which the analysis should be done. Even average computer users are able to interact with the Windows. ![]() When compared to popular chimera screening software the B2C2 performed as well or better while dramatically decreasing the amount of time required generating and screening results. The graphical user interface (GUI) is also able to batch process multiple files. Meeting the needs of basic scientists who are venturing into high-throughput microbial diversity studies such as those based upon pyrosequencing and specifically providing a solution for Windows users, the B2C2 software is designed to be able to accept files containing large multi-FASTA formatted sequences and screen for possible chimeras in a high throughput fashion. ![]() Technologies like Roche 454 FLX and Titanium have been adapted over the past years especially with the introduction of bacterial tag-encoded FLX/Titanium amplicon pyrosequencing methodologies to produce over one million 250-600 bp 16S rRNA gene reads that need to be depleted of chimeras prior to downstream analysis. The existing chimera detection programs are not specifically designed for "next generation" sequence data. ![]()
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